rabbit rag1 antibody  (Cell Signaling Technology Inc)

Journal: Chemico-biological interactions

Article Title: Arsenic Sulfide Enhances Radiosensitivity in Rhabdomyosarcoma via Activating NFATc3-RAG1 Mediated DNA Double Strand Break (DSB).

doi: 10.1016/j.cbi.2024.111149

Figure Lengend Snippet: Fig. 4. A - NAC can reverse the transfer of NFATc3 from nucleus to cytoplasm caused by As4S4 combined with radiotherapy. RMS A-673 cells were treated with 2 Gy radiation, 1 μM As4S4, and combination therapy，respectively. The expressions of NFATc3 was detected by Western blot 24 h later. B - shC3-1 and shC3-2 were effectively knocked down, Western blot assay were used to evaluate expression levels of NFATc3, RAG1, and γ-H2AX. C - Western blot analysis of γ-H2AX expression changes in RMS A-673 cells after transfection with (shC3-1 + shRAG1-1), shC3-1, and shRAG1-1, respectively.

Article Snippet: Antibodies were listed as follows, rabbit NFATc3 antibody (ABclonal, China), rabbit RAG1 antibody (Novus, USA), rabbit p53 antibody (CST, USA), rabbit phospho-histone H2AX antibody (ABclonal, China), rabbit β-Tubulin antibody (Novus, USA), rabbit PCNA antibody (Novus, USA), and rabbit GADPH antibody (CST, USA).

Techniques: Western Blot, Expressing, Transfection

Journal: Chemico-biological interactions

Article Title: Arsenic Sulfide Enhances Radiosensitivity in Rhabdomyosarcoma via Activating NFATc3-RAG1 Mediated DNA Double Strand Break (DSB).

doi: 10.1016/j.cbi.2024.111149

Figure Lengend Snippet: Fig. 3. As4S4 enhances radiosensitivity via activating NFATc3-RAG1 mediated DSB in RMS cells. All samples were run in triplicate. A - RMS cells were treated with radiotherapy, As4S4, and combination therapy, respectively. Western blot assay and qPCR were used to evaluate expression levels of NFATc3 and RAG1. B - RMS cells were treated with radiotherapy, As4S4, and combination therapy, respectively. Western blot assay and qPCR were used to evaluate expression levels of p53 and γ-H2AX. C - RMS cells were treated with radiotherapy, As4S4, and combination therapy, respectively. Immunofluorescence assays for the expression of γ-H2AX. Left, γ-H2AX (red); middle, DAPI (blue); right, γ-H2AX and DAPI merged.

Article Snippet: Antibodies were listed as follows, rabbit NFATc3 antibody (ABclonal, China), rabbit RAG1 antibody (Novus, USA), rabbit p53 antibody (CST, USA), rabbit phospho-histone H2AX antibody (ABclonal, China), rabbit β-Tubulin antibody (Novus, USA), rabbit PCNA antibody (Novus, USA), and rabbit GADPH antibody (CST, USA).

Techniques: Western Blot, Expressing, Immunofluorescence

Journal: Chemico-biological interactions

Article Title: Arsenic Sulfide Enhances Radiosensitivity in Rhabdomyosarcoma via Activating NFATc3-RAG1 Mediated DNA Double Strand Break (DSB).

doi: 10.1016/j.cbi.2024.111149

Figure Lengend Snippet: Fig. 6. A, B - IHC images (NFATc3 and RAG1) in RMS tissues of in vivo xenograft models (40X). C, D - Histograms of NFATc3 and RAG1 in qPCR experiments.

Article Snippet: Antibodies were listed as follows, rabbit NFATc3 antibody (ABclonal, China), rabbit RAG1 antibody (Novus, USA), rabbit p53 antibody (CST, USA), rabbit phospho-histone H2AX antibody (ABclonal, China), rabbit β-Tubulin antibody (Novus, USA), rabbit PCNA antibody (Novus, USA), and rabbit GADPH antibody (CST, USA).

Techniques: In Vivo

Journal: Chemico-biological interactions

Article Title: Arsenic Sulfide Enhances Radiosensitivity in Rhabdomyosarcoma via Activating NFATc3-RAG1 Mediated DNA Double Strand Break (DSB).

doi: 10.1016/j.cbi.2024.111149

Figure Lengend Snippet: Fig. 7. NFATc3 and RAG1 are independent prognostic factors for RMS patients. A, B - IHC images of NFATc3 and RAG1 in RMS tissues (40X). C - The Kaplan-Meier survival curves of the overall survival of two groups defined as low or high expression (NFATc3 and RAG1) in 59 RMS patients. D - Boxplots of expression (NFATc3 and RAG1) between tumor and adjacent normal tissues. E - Nomogram of the prediction model. F. ROC curves predicting 5-year survival of NFATc3-RAG1 based prediction model, TNM staging, and Risk level.

Article Snippet: Antibodies were listed as follows, rabbit NFATc3 antibody (ABclonal, China), rabbit RAG1 antibody (Novus, USA), rabbit p53 antibody (CST, USA), rabbit phospho-histone H2AX antibody (ABclonal, China), rabbit β-Tubulin antibody (Novus, USA), rabbit PCNA antibody (Novus, USA), and rabbit GADPH antibody (CST, USA).

Techniques: Expressing

Journal: Chemico-biological interactions

Article Title: Arsenic Sulfide Enhances Radiosensitivity in Rhabdomyosarcoma via Activating NFATc3-RAG1 Mediated DNA Double Strand Break (DSB).

doi: 10.1016/j.cbi.2024.111149

Figure Lengend Snippet: Fig. 8. Schematic figure describing that As4S4 enhances radiosensitivity in RMS via activating NFATc3-RAG1 mediated DSB.

Article Snippet: Antibodies were listed as follows, rabbit NFATc3 antibody (ABclonal, China), rabbit RAG1 antibody (Novus, USA), rabbit p53 antibody (CST, USA), rabbit phospho-histone H2AX antibody (ABclonal, China), rabbit β-Tubulin antibody (Novus, USA), rabbit PCNA antibody (Novus, USA), and rabbit GADPH antibody (CST, USA).

Techniques:

Journal: PLoS ONE

Article Title: Ambient Oxygen Promotes Tumorigenesis

doi: 10.1371/journal.pone.0019785

Figure Lengend Snippet: A. Changes in reduced (GSH) and oxidized (GSSG) glutathione levels show increased antioxidant capacity in blood of mice after chronic adaptation to the 10% oxygen condition. Data shown as mean ± SEM, with n = 4. B. Decreased ROS levels measured by DCF FACS in cells isolated from the thymus of mice in 10% oxygen. Data shown as mean ± SEM, with n = 5. C. Representative images of decreased oxidative DNA damage detected by avidin-FITC (Av-FITC) staining for 8-oxoG in thymus tissue from p53−/− mouse exposed to 10% versus 21% oxygen for 2 to 4 wk. Nuclei counterstaining with DAPI show similar densities in the tissue. Scale bar, 100 µm (originally 63× magnification). D. Decreased oxidative DNA damage quantified by 8-oxoG enzyme-linked immunosorbent assay (ELISA) in thymus tissue from p53−/− mice exposed to 10% compared to 21% oxygen. Absolute value of 8-oxoG (pg/µg genomic DNA) shown as mean ± SEM, with n = 3. E. Increased relative telomere length measured by RT-PCR of genomic DNA from thymus tissue of p53−/− mice exposed to 10% versus 21% oxygen. Data shown as mean ± SEM, with n = 3. F. Decreased RAG1 protein level measured by western blotting in 10% versus 21% oxygen. Samples shown are from two separate animals in each oxygen condition and β-actin serves as protein loading control.

Article Snippet: Antibodies used in the study are as follows: Rabbit polyclonal RAG1 (K-20, Santa Cruz Biotechnology); monoclonal phospho-S-139 histone H2AX (γ-H2AX, clone JBW301, Millipore); monoclonal β-actin (AC-15, Sigma); rabbit monoclonal β-catenin (6B3) and cyclin D1 (DCS6, Cell Signaling).

Techniques: Isolation, Avidin-Biotin Assay, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control